AMELIORATIVE EFFECT OF BERBERIS VULGARIS FRUIT EXTRACT AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN ALBINO RATS

  • Lubna Faisal Liaquat National Hospital and Medical College, Karachi Pakistan
  • Zia -Ul- Islam Liaquat National Hospital and Medical College, Karachi Pakistan
  • Saima Athar Liaquat National Hospital and Medical College, Karachi Pakistan
  • Sadia Iqbal Dow University of Health Sciences, Karachi Pakistan
  • ```Fatima Rehman Bahria University Medical and Dental College, Karachi Pakistan
  • Syed Shariq Ullah Liaquat National Hospital and Medical College, Karachi Pakistan
Keywords: Berberis vulgaris, Gentamicin, Kidney, Nephrotoxicity

Abstract

Objective: To observe the nephroprotective role of berberis vulgar is on renal parenchyma against microscopic and morphometric changes induced by Gentamicin in albino rats.

Study Design: Lab based experimental study.

Place and Duration of Study: It was conducted in Baqai Medical University in collaboration with department of Anatomy Liaquat National Hospital and Medical College, from Jan to Jul 2017.

Methodology: A total of 40 male adult albino rats were used in the study. Four groups were made. Each group contained 10 rats. Group A was a control group, group B received only berberis vulgaris fruit extract orally per day for 21 days, group C received Gentamicin 100 mg/kg/day intraperitonially daily for 21 days. Group D received Gentamicin 100 mg/kg/day intraperitonially along with berberis vulgaris fruit extract 100 mg/kg/day orally. Both kidneys were removed. H&E and PAS stains were used for observing histological alterations and protective role of berberis vulgaris fruit extract.

Results: Glomerulus and proximal convoluted tubules were observed histologically in all 4 groups. Microscopy of group B showed parameters nearly similar to control group. Microscopy of group C showed significant derangement in all parameters when compared with control group. Group C showed decrease glomerular, proximal convoluted tubular count was noted. Glomerular diameter increases and there was glomerular hypertrophy and tubular necrosis. Microscopy of group D showed significant improvement due to berberis vulgaris which restored normal renal architecture.

Conclusion: Berberis vulgaris has a nephroprotective effect and it can be used as a new medicine against nephrotoxic drugs like Gentamicin.

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Author Biographies

Zia -Ul- Islam, Liaquat National Hospital and Medical College, Karachi Pakistan

Professor and Head department of Anatomy

Saima Athar, Liaquat National Hospital and Medical College, Karachi Pakistan

Associate Professor department of Anatomy

Sadia Iqbal, Dow University of Health Sciences, Karachi Pakistan

Assistant professor department of Anatomy

```Fatima Rehman, Bahria University Medical and Dental College, Karachi Pakistan

Lecturer department of Anatomy

Syed Shariq Ullah, Liaquat National Hospital and Medical College, Karachi Pakistan

Lecturer department of Anatomy

Published
2021-04-30
How to Cite
Faisal, L., Islam, Z., Athar, S., Iqbal, S., Rehman`., & Ullah, S. (2021). AMELIORATIVE EFFECT OF BERBERIS VULGARIS FRUIT EXTRACT AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN ALBINO RATS. PAFMJ, 71(2), 676-80. https://doi.org/10.51253/pafmj.v71i2.3505
Section
Original Articles