SCREENING AND IDENTIFICATION OF RED CELL ALLOANTIBODIES IN MULTIPAROUS WOMEN, AN INSTITUTION BASED STUDY
Keywords:Blood group systems, Hemolytic Disease of Fetus and Newborns (HDFN), Hemolytic Transfusion Reactions (HTR), Red cell alloantibodies
Objective: To screen and identify red cell alloantibodies in multiparous women by red cell panels.
Study Design: Cross sectional study.
Place and Duration of Study: Army Medical College and Armed Forces Institute of Transfusion in collaboration with Pak Emirates Military Hospital Rawalpindi, duration of study was from 1st Jan 2018 to 31st Aug 2018.
Material and Methods: Permission from Institutional Ethical Committee was obtained and blood samples of 200 multiparous women were taken from PEMH Rawalpindi, selected by non-probability consecutive sampling technique. After preliminary workup of ABO and Rh D blood grouping, samples were screened by 3 cell panel to detect presence of red cell alloantibodies and positive results were further identified by 11 cell panel following manufacturer’s instructions.
Results: Two Hundreds (200) multiparous women were screened for presence of red cell alloantibodies. The frequency of alloantibodies in multiparous women was 3% (6/200). Most prevalent alloantibody identified was anti-D having frequency of 1.5% (3/6), while other alloantibodies included anti e 0.5% (1/6), Anti K 0.5% (1/6) and insignificant antibody 0.5% (1/6).
Conclusion: Our study confirmed that Rh antibodies were most prevalent alloantibodies, out of which anti D was the most frequent alloantibody in Pakistani population while anti e and anti K were present in relatively lower frequency.