The Detection of BRAF-V600E Mutation in Hairy Cell Leukaemia by Polymerase Chain Reaction and Study of its associated Clinicohaematological Parameters

  • Muhammad Ijaz Iqbal Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
  • Helen Mary Robert Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
  • Nabeela Khan Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
  • Rafia Mahmood Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
  • Ayesha Khurshid Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
  • Saleem Ahmed Khan Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Keywords: BRAF V600E mutation, B-cell lymphoproliferative, Complete blood counts, Chronic lymphocytic leukemia, Monocytopenia, Pallor

Abstract

Objective: To contemplate the occurrence of a rare mutation (BRAF V600E) in the patients having Hairy cell leukemia in our setting, which wouldserve asuseful analytic and diagnostic criteria.

Study Design: Analytical cross sectional study.

Place and Duration of Study: Armed Forces Institute of Pathology Rawalpindi Pakistan, from Oct 2017 to Oct 2018.

Methodology: A sample of 36 patients with a suspicion of Hairy cell leukemia were included in this investigation. Complete blood counts, bone marrow examination (trephine biopsy and aspiration), cyto-chemistry and staining of the patients suspected to have Hairy cell leukemia was performed. PCR was done for detection of BRAF V600E mutation.

Results: 29 (80.5%) cases showed a positive BRAF mutational analysis and 7 (19.4%) cases showed a negative result.

Conclusion: Almost all the cases of classic Hairy cell leukemia had BRAF V600 E mutation.Isolating this mutation can be used as a regular tool in detection of disease and monitoring disease progression and relapse.

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Published
2022-06-02
How to Cite
Iqbal, M., Robert, H., Khan, N., Mahmood, R., Khurshid, A., & Khan, S. (2022). The Detection of BRAF-V600E Mutation in Hairy Cell Leukaemia by Polymerase Chain Reaction and Study of its associated Clinicohaematological Parameters. Pakistan Armed Forces Medical Journal, 72(SUPPL-2), S178-81. https://doi.org/10.51253/pafmj.v72iSUPPL-2.3083
Section
Original Articles