EFFECTS OF SLEEP DEPRIVATION ON THE EPITHELIAL HEIGHT OF THE PROSTATIC ACINI IN RATS AND THE PROTECTIVE EFFECTS OF OMEGA 3 FATTY ACIDS
Epithelial Height of The Prostatic Acini
Objective: To study the protective role of omega 3 fatty acids (omg 3 FAs) on the histomorphological changes in the height of the prostatic epithelium in rats induced by sleep deprivation.
Study Design: Lab based randomized control trial.
Place and Duration of Study: The study was conducted at Anatomy Department, Army Medical College, Rawalpindi, in collaboration with National Institute of Health (NIH), Rawalpindi for duration of one year, from Nov 2014 to Nov 2015.
Material and Methods: Thirty male Sprague Dawley rats, 3-4 months of age with average weights of 200-300 grams (gm) were divided in three groups each having 10 rats. Group A served as control with standard lab diet and regular sleep -wake cycle. Group B was subjected to sleep deprivation of 16 hours followed by a sleep window of 8 hrs daily for 2 months and group C was administrated with omg 3 fatty acids (FAs) and was sleep deprived as group B for 2 months. At the end of the experimental period rats were anesthetized and their blood sample was drawn for hormonal assay. They were dissected and the prostate gland was removed and fixed in 10 percent formalin. Five micrometer (µm) sections were obtained after tissue processing and stained with haematoxylin and eosin (H&E) for histological study.
Results: Microscopic examination revealed that the epithelium of glandular acini was columnar in group A. Marked decrease in the height of cells was observed in group B whereas the epithelium was nearly cuboidal in group C.
Conclusion: It was concluded that sleep deprivation had deleterious effects on the epithelium of the prostatic acini and that Omega 3 fatty acids had a protective effect on the epithelium of the prostatic acini.